Description
Recombinant human NEK5 (1-508) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_199289.
NEK5 or NIMA (never in mitosis gene a)-related kinase 5 is a serine/threonine kinase that is involved in cell cycle regulation. NEK5 has been shown to interact with NEK6 and NEK5 are required for the ibo1/nek6 ectopic outgrowth phenotype in epidermal cells (1). NEK6 interacts and co-localizes with NEK5 in a transient expression assay. The ibo1-3 mutation markedly increases the interaction between NEK6 and NEK5. NEK6 forms heterodimers with NEK5 to regulate cortical microtubule organization possibly through the phosphorylation of beta-tubulins. The NEK5 gene is conserved in chimpanzee, cow, mouse, rat, and chicken